| The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) are important in the regulation of growth and differentiation of eukaryotic cell. Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of tyrosine 416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of tyrosine 527 in the C-terminal tail by Csk renders the enzyme less active. |
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| Catalog # | S6504-36A |
| Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. |
| Immunoprecipitation: 1:100 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 3A6 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. |
| Purity | Ascites |
| Immunogen | Synthetic peptide corresponding to residues surrounding Tyr416 of human Src (KLH). |
| Specificity | Recognizes endogenous levels of human Src only when tyrosine 416 is dephosphorylated at ~60kD. Recognizes other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
