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Anti -Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)
Anti -Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| The Src family of protein tyrosine kinases is important in the regulation of growth and differentiation of eukaryotic cells (1,2). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of Tyr527 in the C-terminal tail by Csk renders the enzyme less active (3,4). | | | Catalog # | S6504-36B | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunohistochemistry (Paraffin): 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr527 of human Src. | | Specificity | Recognizes human Src only when phosphorylated at Tyr527. It may crossreact with other Src family members Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites. Species Crossreactivity: Mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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