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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Src, CT (pp60Src, pp60c-Src, ASV)

Anti -Src, CT (pp60Src, pp60c-Src, ASV)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).
Catalog #S6504-40B
ApplicationsSuitable for use in Western Blotting, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blotting: 1:1,000-5,000 (60kD)
Immunohistochemistry:1:50-100
Immunocytochemistry: 1:250-500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k377
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues to the C-term of human Src.
SpecificityRecognizes human Src.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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