Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Src, NT (pp60Src, pp60c-Src)

Anti -Src, NT (pp60Src, pp60c-Src)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse E B
Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kindase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an “open”, active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organizations, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.
Catalog #S6504-40M
ApplicationsSuitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5ug/ml for HRPO/ECL detection.
Blocking buffer: Casein/Tween 20
ELISA: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control (included)A0001-50B A431 cell lysate, untreated
Add 100ul 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Western Blot: 20ul/lane (mini gel) for HRPO/ECL detection
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D774
HostMouse
FormSupplied as a liquid in PBS, PEG, sucrose, 0.09% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to a portion of N-terminal Src conjugated to hemocyanin.
SpecificityRecognizes the N-terminus of Scr at ~60kD. Species Crossreactivity: human, mouse, rat, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links