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You are here:Home » Antibodies » Abs to Disease Markers » Anti -SSA1/Ro-52 (Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

Anti -SSA1/Ro-52 (Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro,
RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IC
TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52kD Ro Protein/Ro52, and RING finger protein 81) is a 52-56kD member of the RING finger-B-box- coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475aa in length. It contains one E3 ligase RING finger domain (aa16-55), a B-Box type zinc-finger region (aa92-123), a coiled-coil region (aa128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa195-293, human TRIM21 exhibits 72aa identity with mouse TRIM21.
Catalog #S6600-65C1
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 8-25ug/ml using immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20ng/ml of recombinant human IFNN-gamma.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No11n177
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa195-293 from human TRIM21 expressed in E. coli. (P19474).
SpecificityRecognizes human TRIM21. Does not react with recombinant human TRIM, TRIM5, TRIM5a, TRIM32, TRIF (aa29-204) or TRIF (aa474-618).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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