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You are here:Home » Antibodies » Antibodies-Disease Markers » Anti -SSA1/Ro-52 (Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

Anti -SSA1/Ro-52 (Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro,
RO52, RNF81, Tripartite Motif Protein 21, TRIM21)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IC
TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52kD Ro Protein/Ro52, and RING finger protein 81) is a 52-56kD member of the RING finger-B-box- coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475aa in length. It contains one E3 ligase RING finger domain (aa16-55), a B-Box type zinc-finger region (aa92-123), a coiled-coil region (aa128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa195-293, human TRIM21 exhibits 72aa identity with mouse TRIM21.
Catalog #S6600-65C1
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 8-25ug/ml using immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20ng/ml of recombinant human IFNN-gamma.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No11n177
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa195-293 from human TRIM21 expressed in E. coli. (P19474).
SpecificityRecognizes human TRIM21. Does not react with recombinant human TRIM, TRIM5, TRIM5a, TRIM32, TRIF (aa29-204) or TRIF (aa474-618).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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