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You are here:Home » Antibodies » Abs to Stem Cell Research » Anti -Stage Specific Embryonic Antigen 4 (SSEA4)

Anti -Stage Specific Embryonic Antigen 4 (SSEA4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E IH FC IF
Expression of SSEA4 is down-regulated following differentiation of human tetracarcinoma stem (EC) cells. In contrast, the differentiation of murine tetracarcinoma stem (EC) cells and embryonic stem (ES) cells may be accompanied by an increase in SSEA4 expression.
Catalog #S6610-10
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended DilutionFlow Cytometry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlPluripotent human embryonic stem cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG3
Clone No5C1
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M PBS, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A chromatography.
ImmunogenHuman embryonal carcinoma cell line 2102Ep.
SpecificityRecognizes Stage-specific embryonic antigen 4 (SSEA4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. The differentiation of murine EC. and ES cells may be accompanied by an increase in SSEA-4. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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