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You are here:Home » Antibodies » Abs to Stem Cell Research » Anti -Stage Specific Embryonic Antigen 4 (SSEA4)

Anti -Stage Specific Embryonic Antigen 4 (SSEA4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.1-4
Catalog #S6610-11
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the human embryonal carcinoma cell line 2102 Ep.
ApplicationsFlow Cytometry, Immunohistochemistry
Recommended DilutionImmunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10ug/mL in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10% normal donkey serum and 1% BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Redô coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10ug/mL.
Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG3
Clone No8H218
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in TBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman embryonal carcinoma cell line 2102 Ep
SpecificityRecognizes a carbohydrate epitope of human SSEA-4. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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