You are here:Home
»
Antibodies
»
Abs to Stem Cells
»
Anti -Stage Specific Embryonic Antigen 4 (SSEA4)
Anti -Stage Specific Embryonic Antigen 4 (SSEA4)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.1-4 | | | Catalog # | S6610-11 | | Hybridoma | Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the human embryonal carcinoma cell line 2102 Ep. | | Applications | Flow Cytometry, Immunohistochemistry | | Recommended Dilution | Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10ug/mL in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10% normal donkey serum and 1% BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red™ coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10ug/mL. | | Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG3 | | Clone No | 8H218 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder in TBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Human embryonal carcinoma cell line 2102 Ep | | Specificity | Recognizes a carbohydrate epitope of human SSEA-4. Species Crossreactivity: Mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
