Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 2.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
An 18 amino acid peptide located near the C-terminal region of human STEAP1. Species Sequence Homology: porcine
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human STEAP1. Does not cross-react with other STEAP proteins. Species Crossreactivity: mouse and rat
References
1. Hubert, R.S., et al (1999) STEAP: A prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc Natl Acad Sci U S A. 96:14523-14528 |2. Alves, P.M., et al (2006) STEAP, a prostate tumor antigen, is a target of human CD8+ T cells. Cancer Immunol. Immunother. 55:1515-1523
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