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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -STABILIN-1-INTERACTING CHITINASE-LIKE PROTEIN, (SI-CLP)

Anti -STABILIN-1-INTERACTING CHITINASE-LIKE PROTEIN, (SI-CLP)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B FC
SI-CLP is a secretory protein which is up-regulated in response to interleukin-4 and dexamethasone in activated macrophages. SI-CLP is also expressed by T- and B-lymphocytes, and by cells of monocytic and epithelial origin. SI-CLP is the ligand for stabilin-1, an intracellular sorting receptor which sorts SI-CLP into late endosomes and secretory lysosomes in activated macrophages.
Catalog #S7950-56A
ApplicationsSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:100 Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.
Western Blot: Detects bands of approximately 33 and 39kD in U937 cell lysates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9A206
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSI-CLP fragment corresponding to amino acids 24-73 expressed as a GST fusion protein in E. coli.
SpecificityDetects human Stabilin-interacting chitinase like protein (SI-CLP), a novel member of the Glyco-18- domain containing protein family.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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