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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 1, CT (Signal Transducer and Activator of Transcription 1)

Anti -STAT 1, CT (Signal Transducer and Activator of Transcription 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Catalog #S7969-05
Positive Antigen ControlJurkat cell lysate. Use 20ug per lane for minigels.
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation.
Western Blotting 0.52ug/ml detects STAT1 in RIPA lysates from human Jurkat, mouse CTLL and rat L6 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation4mg immunoprecipitated STAT1 from 500mg of human Jurkat RIPA lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage , store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 250ul of 0.07M Tris-glycine, 0.105M sodium chloride, 0.035% sodium azide, glycerol added to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenGST fusion-protein containing the carboxy terminal 36 amino acids of human STAT1a. This sequence is missing in the b isoform.
SpecificityRecognizes the STAT1 protein, Mr 91kDa. of human, mouse and rat. Other species crossreactivity is unknown.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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