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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 1 (Signal Transducer and Activator of Transcription 1)

Anti -STAT 1 (Signal Transducer and Activator of Transcription 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP
Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-alpha and IFN-gamma (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1alpha (91kD) and the splice variant Stat1beta/Stat2 (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors (5).
Catalog #S7969-05B
ApplicationsSuitable for use in ELISA, Western Blot and Immunopreipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3A28
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
PurityAscites
ImmunogenSynthetic peptide (KLH coupled).
SpecificityRecognizest otal Stat1 levels independent of phosphorylation. Recognizes Stat1alpha (91kD) and Stat1beta (84kD). It does not appreciably crossreact with the other Stat proteins. Species Crossreactivity: Human and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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