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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)

Anti -STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IC FC IF
Stat1, while activated in response to a large number of ligands, appears to be essential for responsiveness to IFN-alpha and IFN-gamma. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding. Stat1 has two isoforms, Stat1alpha (91 kD) and the splice variant Stat1beta/Stat2 (84 kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors.
Catalog #S7969-05C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunocytochemistry (ABC and IF). Other applications not tested.
Recommended DilutionsImmunoprecipitation: 1:100
Flow Cytometry: 1:50
Western blot: 1:1000
Immunocytochemistry (ABC): 1:500
Immunocytochemistry (IF): 1:50
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.04mg/ml
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr701 of human Stat1.
SpecificityDetects endogenous levels of human Stat1 only when phosphorylated at Tyr701. Detects phosphorylated tyrosine 701of p91 Stat1 and also the p84 splice variant. Does not crossreact with the corresponding phospho-tyrosines of other Stat proteins.
Species crossreactivityMouse and rat. Species sequence homology: Bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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