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You are here:Home » Antibodies » Antibodies-Transcription Factors, STAT » Anti -STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1)

Anti -STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The signal transducer and activator of transcription (STAT) family of transcription factors is composed of seven family members (STATs 1, 2, 3, 4, 5A, 5B and 6) that are involved in many cell processes (apoptosis, anti-apoptosis, cell differentiation and proliferation) depending upon the cell type. STATs are activated by ligand binding of mostly cytokine receptors, that receptor associating with a Janus kinase (JAK) family member that phosphorylates a STAT protein causing it to translocate to the nucleus, where it can bind to DNA and regulate the transcription of particular genes. STAT1 serine 727 can be phosphorylated by p38, PKC and CaMKII and has been found to be required for maximum transcriptional activity.
Catalog #S7969-07A
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution to be determined by the researcher.
Positive ControlHEK293 cells treated with UV light; 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human STAT1 that contains serine 727.
SpecificityRecognizes human and mouse STAT1 when phosphorylated at Ser727.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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