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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 1, phosphorylated (Alexa FluorŪ 488)

Anti -STAT 1, phosphorylated (Alexa FluorŪ 488)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified FC
Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-alpha and IFN-gamma (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1apha (91kD) and the splice variant Stat1beta (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is phosphorylated through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway at Ser727 in response to IFN-alpha and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
Catalog #S7969-10A
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10, Add 10ul to 500,000 cells in 90ul PBS, 0.5% BSA.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.
Note: We do not recommend adding glycerol as a cryopreservative.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H64
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. Labeled with Alexa Fluor® 488. Fluorophore/Protein ratio: 2-6.
PurityPurified prior to conjugation
ImmunogenSynthetic phospho peptide (KLH-coupled) corresponding to residues surrounding Tyr701 of human Stat1.
SpecificityRecognizes endogenous levels of human phospho(Tyr701)Stat1. Also recognizes p91 Phospho(Tyr701)Stat1 and p84 Phospho-Stat1 splice variants. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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