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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 1, alpha (p91) (Signal Transducer and Activator of Transcription 1, alpha)

Anti -STAT 1, alpha (p91) (Signal Transducer and Activator of Transcription 1, alpha)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Stimulation of responsive cells with IFN a/b induces the formation of a transcription complex termed ISGF3. This complex binds to the interferon-stimulated response element (ISRE) and thereby activates the transcription of responsive genes. The ISGF3 complex consists of tyrosine phosphorylated STAT1a, STAT1b (p84) and STAT2 proteins, as well as a 48kD (p48) DNA binding protein that specifically binds to the IFN-stimulated response element. Formation of this complex and its migration into the nucleus is dependent upon tyrosine phosphorylation of STAT1a/b and STAT2. Stimulation of cells with IFN-g results in tyrosine phosphorylation of STAT1a (p91) but not of STAT2. Phosphorylation of STAT1a results in homodimerization and migration to the nucleus where it binds to the IFN-g activated site (GAS). The STAT1a and STAT1b isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1b lacks 38 carboxy-terminal amino acids. Contained within these 38 terminal amino acids of STAT1a is a critical serine residue (Ser-727) whose phosphorylation is required for maximal transcription induced by IFN-g.
Catalog #S7969-60
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.1-1.0ug/ml
Western Blot: 1-2ug/ml
Immunohistochemistry (FFPE): 0.1-1.0ug/ml. Heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 is required prior to staining.
Optimal dilutions to be determined by the researcher.
Positive ControlWestern Blot: A431 cell lysate
Immunohistochemistry (FFPE): Human tonsil and kidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G273
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide located at the C-terminus of the human STAT1 protein. The sequence is identical in mouse STAT1 protein.
SpecificityRecognizes human STAT1a (p91) protein and does not recognize the STAT1b (p84) protein. Does not appear to crossreact with other STAT family members. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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