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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 2, phosphorylated (Tyr689) (Signal Transducer and Activator of Transcription 2)

Anti -STAT 2, phosphorylated (Tyr689) (Signal Transducer and Activator of Transcription 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
STAT (for signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, which are thought to be essential for their selective activity. Seven STAT proteins have been described (STAT1a, STAT1b, STAT2, STAT3, STAT4, STAT5, and STAT6) and range in molecular weight from 84-113kD. Interferon-a promotes phosphorylation of STAT1 and STAT2, which then form heterodimers that interact with the 48kD DNA binding protein ISGF3. This complex moves to the nucleus where it binds to interferon-stimulated response elements and activates transcription of specific genes. In contrast to interferon-a signaling, interferon-g only induces phosphorylation of STAT1a or b. STAT proteins may be involved in the intracellular signaling of many cytokines and growth factors via the activation of gene expression.
Catalog #S7970-80
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:500-1:2000 detected phosphorylated STAT2 in HeLa whole cell RIPA lysate stimulated with interferon gamma (0.3ng/ml) for 16-20 hours and then treated with interferon alpha (2000 units/ml) for 20 minutes. HeLa whole cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7970-80 (1:200). Proteins were visualized using a goat anti-rabbit (HRP) secondary and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa683-691, C-EEQRK[pY]LKH of mouse STAT2 (KLH).
SpecificityRecognizes mouse STAT2 phosphorylated at Tyr689, Mr130kD.
Species CrossreactivityHuman. Species Sequence Homology: human (7/9 aa)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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