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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

Anti -STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response
Factor, APRF)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4).
Catalog #S7971-01K
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blotting:1:1,000 (92kD)
Immunohistochemistry: 1:50
Immunocytochemistry: 1:100
Immunoprecipitation: 1:20 1ug/mg of total cell protein (~10e7 cells)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k380
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues surrounding Ser727 of human Stat3.
SpecificityRecognizes human Stat3. Detects Stat3 without phosphorylation on Serine 727. It does not detect S727-phosphorylated Stat3. Predicted to cross-react with bovine, and horse, based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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