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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)

Anti -STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response
Factor, APRF, FLJ20882, MGC16063)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
Catalog #S7971-01U2
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry (paraffin): 1:50
Immunocytochemistry: 1:100
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i213
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Ser727 of human STAT3.
SpecificityRecognizes human STAT3 without phosphorylation on Ser727. It does not detect Ser727-phosphorylated STAT3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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