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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 4 (Signal Transducer and Activator of Transcription 4)

Anti -STAT 4 (Signal Transducer and Activator of Transcription 4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site
(GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin-12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. Perhaps not surprisingly, STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study has suggested that tyrosine and serine phosphorylation of STAT4 can be induced by both IL-12 and IFN-alpha.
Catalog #S7972-03
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.1-1.0ug/ml
Immunoprecipitation: 5ug
Gel Mobility Shift Assay: 1-5ug/ml
Western Blot: 2-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlMouse testis lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from the extreme carboxy-terminus of murine STAT4.
SpecificityRecognizes mouse the STAT4 protein. Crossreactivity with other STAT family members has not been detected. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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