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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5, phosphorylated (Tyr694)

Anti -STAT 5, phosphorylated (Tyr694)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin (1,2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the a- and b-isoforms of Stat5 (3).
Catalog #S7972-07E
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blotting: 1:1,000 (90kD )
Immunohistochemistry: 1:50
Immunocytochemistry: 1:50
Immunoprecipitation: 1:100 1ug/mg of total cell protein (~10e7 cells)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k383
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic phospho
peptide corresponding to residues surrounding Tyr694 of human Stat5.
SpecificityRecognizes human Stat5 when phosphorylated at Tyr694.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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