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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

Anti -STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by src upon erythropoietin stimulation (3). Stat5 has been found to be constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5alpha and beta are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells (6).
Catalog #S7972-07F
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (paraffin): 1:600. Antigen retrieval with citrate buffer is recommended.
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H67
HostRabbit
SourceHuman
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Tyr694 of Stat5. Species Sequence Homology: Bovine, monkey, rat; 100%.
SpecificityRecognizes endogenous levels of human Stat5 protein when phosphorylated at Tyr694. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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