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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

Anti -STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation on residue Tyr694 is obligatory for Stat5 activation (1,2). Stat5 has been found to be constitutively active in some leukemic cell types (3). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (4). Stat5alpha and-beta are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells (5).
Catalog #S7972-07H
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5i114
HostMouse
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, < 0.02% sodium azide, 50% glycerol.
PurityAscites
ImmunogenSynthetic phosphopeptide corresponding to residues around tyrosine 694 of mouse Stat5 (KLH coupled).
SpecificityDetects endogenous levels of Stat5 alpha and Stat5 beta only when phosphorylated at tyrosine 694. It does not crossreact with the corresponding phosphotyrosines of other Stat proteins. Species Crossreactivity: human. Predicted to cross-react with rat based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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