STAT 5, phosphorylated

Clone Type

Monoclonal

Host

Mouse

Source

Mouse

Swiss Prot

P42230

Isotype

IgG1

Clone Number

9L774

Grade

Affinity Purified

Applications

E IF IHC WB

Crossreactivity

Hu Mo

Accession #

NP_035618

Gene ID

20850

Shipping Temp

Blue Ice

Storage Temp

-20°C

Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.

Applications

Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions

ELISA: 1:20,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 20-40ug/ml Immunofluorescence: 1:50-1:1000 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr694 of mouse STAT5alpha protein. Species Sequence Homology: rat.

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes human STAT 5, phosphorylated at Tyr694. Species Crossreactivity: mouse.

References

1.Engblom,D., Kornfeld,J.W., Schwake,L., Tronche,F., Reimann,A., Beug,H., Hennighausen,L., Moriggl,R. and Schutz,G. (2007) Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 21 (10), 1157-1162.2.Baugh,J.E. Jr., Floyd,Z.E. and Stephens,J.M. (2007) The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes. Obesity (Silver Spring) 15 (3), 583-590. 3.Laurence,A., Tato,C.M., Davidson,T.S., Kanno,Y., Chen,Z., Yao,Z., Blank,R.B., Meylan,F., Siegel,R., Hennighausen,L., Shevach,E.M. and O'Shea,J.J. (2007) Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation. Immunity 26 (3), 371-381. 4.Burchill,M.A., Yang,J., Vogtenhuber,C., Blazar,B.R. and Farrar,M.A. (2007) IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells. J. Immunol. 178 (1), 280-290.

USBio References

No references available

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S7972-07H1 Mouse Anti-STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

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References

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