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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5, phosphorylated (Signal Transducer and Activator of Transcription 5)

Anti -STAT 5, phosphorylated (Signal Transducer and Activator of Transcription 5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
STATs (signal transducers and activators of transcription) were originally identified as DNA-binding proteins essential to interferon IFNa- and IFNg-regulated gene expression. Since then, several additional mammalian STATs, a Drosophila STAT (D-STAT or Marelle), and a STAT-like protein from Dictyostelium discoideum have been recognized. In unstimulated cells STAT proteins exist largely as latent cytoplasmic transcription factors. Treatment of cells with cytokines or certain growth factors results in the tyrosine phosphorylation (via Janus kinases for cytokines) of specific STATs causing them to form homodimers or heterodimers that enter the nucleus. In the nucleus, STAT dimers bind DNA and activate transcription of distinct target genes. Although several different STATs may be activated in response to a given ligand, there is often a striking specificity in the pattern of STAT activation. Mammalian STAT proteins share several conserved structural and functional domains as illustrated below: STAT5 (mammary growth factor) was originally isolated as a tyrosine phosphorylated DNA binding protein from prolactin stimulated mammary tissue and IL-3 stimulated myeloid cells. Subsequently, two highly homologous (~96%) genes, Stat5a and Stat5b, were identified in mouse.7-8 Both STATs are expressed in most tissues of virgin and lactating mice although differential accumulation of STAT5a and STAT5b mRNAs occurs in muscle and mammary tissues. STAT5 activation has been observed in response to IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GM-CSF, TPO, EPO, GH, and prolactin (PRL). STAT5a and STAT5b proteins recognize the GAS (gamma interferon activating sequence) site TTCNNNGAA with the preferred DNA-binding consensus site deduced as TTCC(A>T)GGAA. Western blot analysis of the full-length STAT5a protein, isolated from a variety of cultured cells, indicates that the protein migrates with a molecular weight of ~95kD. C-terminal truncated forms of the STAT5a protein have also been reported.
Catalog #S7972-07J
ApplicationsSuitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
ControlsSTAT5 transfected 293 cells expressing either wild type or inactive JAK1 kinase, A431and K562 cell lysates prepared before and after treatment with epidermal growth factor.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A188
HostMouse
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, containing 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography
ImmunogenA synthetic tyrosine phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of murine STAT5.
SpecificityRecognizes the tyrosine phosphorylated form of STAT5a and STAT5b proteins. It does not cross-react appreciably with the corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine containing proteins. Species sequence homology: human, bovine, sheep, and rat STAT5 proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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