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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5, phosporylated (Tyr699) (Signal Transducer and Activator of Transcription 5)

Anti -STAT 5, phosporylated (Tyr699) (Signal Transducer and Activator of Transcription 5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN- ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.
Catalog #S7972-07N
ApplicationsSuitable for use in Immunocytochemistry and Western Blot. Other applications not tested.
Reommended DilutionImmunocytochemistry: 10ug/ml detected phospho-STAT5 (Y699) in IFNa-treated Daudi cells. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-rabbit IgG in the dark. Between each step, cells were washed with PBS containing BSA.
Western Blot 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a powder lyophilized from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 100ul of sterile PBS containing 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPhosphopeptide containing human STAT5 phosphorylated at the Y699 site
SpecificityDetects human STAT5A phosphorylated at Y694 and STAT5B phosphorylated at Y699. Does not recognize STAT5A/B unphosphorylated at Y694/Y699.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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