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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5a (Signal Transducer and Activator of Transcription 5a)

Anti -STAT 5a (Signal Transducer and Activator of Transcription 5a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
STAT (signal transducers and activators of transcription) can be selectively phosphorylated on tyrosine and serine residues. The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, thought to be essential for their selective activity. STAT5 can be activated by a variety of different agents including prolactin, IL-2, IL-3, IL-7, IL-5, growth hormone and erythropoietin. The highly homologous STAT5A and STAT5B proteins are essential mediators of prolactin and growth hormone effects and have been implicated in physiological functions including cytokine control of apoptosis, growth, reproduction, lactation, immune function, differentiation and somatic growth.
Catalog #S7972-10
Applications Immunoblot Analysis: A 1:2000 dilution detected STAT5A in RIPA lysates from Nb2-SP cells.
Immunoprecipitation 4ul immunoprecipitated STAT5A from 500ug of Nb2-SP RIPA lysate. Immunoblot Analysis: Nb2-SP cell lysate (20ug) was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-STAT5A (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation/Immunoblot Analysis STAT5A was immunoprecipitated from Nb2-SP cell lysate (500ug) using anti-STAT5A (4ul). The reduced immuno-complex was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT5A (1:2000), lane 2, or the secondary antibody alone, lane 1. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemilum-inescence detection system.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormRabbit antiserum
PurityNeat serum
ImmunogenKLH-conjugated, synthetic peptide (LDARLSPPAGLFTSARSSLS) corresponding to amino acids 774793 of mouse STAT5A.
SpecificityRecognizes and specific for mouse STAT5A at Mr ~ 94kDa. Species crossreactivity: rat and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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