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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5a,b (Signal Transducer and Activator of Transcription 5a,b)

Anti -STAT 5a,b (Signal Transducer and Activator of Transcription 5a,b)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum
STAT (signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins are highly conserved in their amino termini but have divergent carboxy termini, which are thought to be essential for their selective activity. STAT5 can be activated by a variety of agents including prolactin, IL-2, IL-3, IL-7, IL-15, growth hormone and erythropoietin. In peripheral blood lymphocytes (PBL), IL-2 rapidly activates STAT5 while phytohemagglutinin activates both STAT3 and STAT5. Evidence indicates that both STAT5A and STAT5B are activated in PBL in vivo. Data show that either Tyr-392 or Tyr-510 of the IL-2Rb is required for IL-2 dependent activation of STAT5. JAK1 and JAK3 may activate each STAT5 isoform by phosphorylation of a single tyrosine residue.
Catalog #S7972-11
Applications Antibody Supershift: An Independent laboratory showed that Anti-STAT5 (A&B) supershifted IL-2 induced complexes in both fresh and preactivated peripheral blood lymphocytes. This antibody also supershifted the complexes induced by IL-2, IL-7 and IL-15, but not those induced by IL-4 and IL-13 in fresh peripheral blood lymphocytes. The specificity of the reaction was confirmed by competition with the immunogenic peptide (Lin, et al., 1995) Experimental conditions must be optimized to achieve the best supershift banding pattern (see Lin, et al., 1993).
Immunoblot Analysis Not recommended.
Immunoprecipitation Not recommended. Immunocytochemistry: Not tested.
Immunohistochemistry Not tested.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormFrozen serum
PurityWhole rabbit antiserum
ImmunogenKLH conjugated synthetic peptide (C-QELQKKAEHQVGEDG), corresponding to amino acids 6680 of Human STAT5 A&B, with the N-terminal cysteine having been added.
SpecificityRecognizes human STAT5 A & B; crossreactivity unknown, although the immunogenic sequence is identical in mouse, rat and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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