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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5a,b, phosphorylated (Ser726/Ser731) (Signal Transducer and Activator of Transcription 5a,b)

Anti -STAT 5a,b, phosphorylated (Ser726/Ser731) (Signal Transducer and Activator of
Transcription 5a,b)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Many hormones and cytokines selectively activate transcription factors of the STAT gene family. STAT5 was originally cloned as a prolactin-stimulated DNA-binding protein, but is also activated by non-lactogenic cytokines in many cell types. Phosphorylation of a conserved tyrosine residue is required for STAT dimerization, nuclear translocation, and specific DNA binding. Serine residues located in a conserved PSP motif of STAT5A (S726) and STAT5B (S731) are also major phosphorylation sites. Interestingly, control of the phosphorylation of the PSP motif differs between the STAT5A (S725) and STAT5B (S730) serine sites.
Catalog #S7972-12
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation/Western Blot Analysis: 1:500-1:2000 dilution detected phosphorylated STAT5A following immunoprecipitation of STAT5A from RIPA lysates of Nb2-Sp cells that had been treated with prolactin; 4ul of S7972-10 was used to immunoprecipitate total STAT5A. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Phosphospecificity was confirmed by dephosphorylation of the STAT5a protein with l-phosphatase.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa722-730, DQAP[pS]PAVC of human STAT5A or aa727-735 of human STAT5B, conjugated to KLH. Species Sequence Homology: bovine and sheep
SpecificityRecognizes human phosphorylated STAT5A (S726) and STAT5B (S731) at ~95kD. Does not recognize STAT5B containing a S731A mutation in lysates of transfected Cos-1 cells, stimulated with prolactin. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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