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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5a,b, phosphorylated (Tyr694/699) (Signal Transducer and Activator of Transcription 5a,b)

Anti -STAT 5a,b, phosphorylated (Tyr694/699) (Signal Transducer and Activator of
Transcription 5a,b)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B Gel
Catalog #S7972-14A
ApplicationsSuitable for use in Western Blot, Peptide Inhibition and Peptide Dot Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000 detects phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF. Non-treated and PDGF treated 3T3/NIH cells were resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-STAT5A/B (Tyr694/699) (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Peptide Inhibition 1uM of the peptide containing the phospho-STAT5A/B (Tyr694/699) modification completely abolished detection of STAT5 by anti-phospho-STAT5A/B (Tyr694/699) in immunoblot analysis of 3T3/NIH cell lysates ( lane 2). 3T3/NIH cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-STAT5A/B (Tyr694/699) (1:500 dilution) or antiphospho- STAT5A/B (Tyr694/699) pre-absorbed with 1uM peptides. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Peptide Dot Blot 1:500-1:2000 detected the peptide containing phospho-STAT5A/B (Tyr694/699).. A dilution series of peptides. 2ul of each dilution was spotted onto PVDF membranes and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:1000 dilution). Peptides were visualized using a goat anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence …DGpYVK… in which pY corresponds to phospho-tyrosine 694 of human STAT5.
SpecificityRecognizes phospho-STAT5A/B (Tyr694/699), Mr 95kD. An unknown protein was also
detected, Mr 200kD. Species CrossreactivityHuman, bovine, rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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