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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5b (Signal Transducer and Activator of Transcription 5b)

Anti -STAT 5b (Signal Transducer and Activator of Transcription 5b)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
STATs were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play central roles in interferon IFNa-and IFNg-regulated gene expression.1 Following the identification of these first two family members, five additional mammalian STAT proteins have been cloned, and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6.1,2 More recently a putative Drosophila STAT protein termed D-STAT or Marelle was described.3,4 All STAT proteins share several conserved structural and functional domains: (1) conserved amino terminal domain (2) DNA binding domain (3) SH3-like region (4) conserved SH2 domain responsible for-recruitment to the receptor interaction with JAKs STAT dimerization (5) conserved tyrosine residue whose phosphorylation is required for dimerization andDNA binding (6) carboxy terminal activation domain. In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding which results in transcriptional activation of distinct target genes(1,2). At least one and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members. Nevertheless, a striking specificity of specific STAT activation is seen in response to individual cytokines (1). STAT5 or MGF (mammary growth factor) was originally isolated by purification of tyrosine phosphorylated DNA binding proteins from prolactin stimulated mammary tissue and from IL-3 stimulated myeloid cells (1,2,5,6). Subsequently, two highly related but distinct Stat5 genes (Stat5a and Stat5b) were identified in mouse (3,7). The amino acid sequences of STAT5a and 5b show ~ 96% sequence similarity, and both proteins are co-expressed in most tissues of both virgin and lactating mice(4,7,8). However, differential accumulation of 5a and 5b mRNAs have been reported for both muscle and mammary tissue. STAT5 has been reported to be activated by multiple cytokines including, IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GMCSF, TPO, EPO, GH, and Prolactin (PRL) (110). Both the STAT5a and 5b proteins recognize the GAS (gamma interferoin activating sequence) site TTCNNNGAA, and the preferred DNA-binding consensus site has been identified as TTCC(A>T)GGAA(1,2). Western analysis of the full-length STAT5b protein isolated from a variety of cultured cells, indicates that this protein migrates with an apparent molecular weight of ~92kD. Carboxy-terminal truncated forms of the STAT5b protein have also been reported (3,9).
Catalog #S7972-15D
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay (EMSA). Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Immunoprecipitation: 5ug
Western Blot: 1-3ug/ml
Electrophoretic Mobility Shift Assay (EMSA): 1-3ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,l
Clone No3G279
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified from mouse ascites by peptide-affinity chromatography.
ImmunogenA peptide corresponding to the unique carboxy-terminus of murine STAT5b coupled to a protein carrier.
SpecificityReacts specifically with the STAT 5b protein and does not exhibit any crossreactivity with the highly related STAT 5a protein. Crossreactivity with other endogenous STAT isoforms has not been observed. Species Crossreactivity: Human, mouse and rat. Reactivity of this antibody with STAT5b has been confirmed by Western blotting using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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