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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5b (Signal Transducer and Activator of Transcription 5b)

Anti -STAT 5b (Signal Transducer and Activator of Transcription 5b)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Catalog #S7972-15F
ApplicationsWestern Blotting: 0.5-2mg/ml detects STAT5B (doublet of 90kD and 92kD) in
human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure.
Immunoprecipitation 4ug immunoprecipitates STAT5B from A431 carcinoma cells and a
previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection.
Positive Antigen Control mouse 3T3 cell lysate. Add 2.5ul of 2-mercaptoethanol
per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.75mg/ml
FormSuppied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenThe antiserum was raised to amino acids 774-787 of human STAT5B (sequence = [C]GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization.
SpecificityRecognizes the 90-92kD forms of STAT5B protein.Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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