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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 5b (Signal Transducer and Activator of Transcription 5b)

Anti -STAT 5b (Signal Transducer and Activator of Transcription 5b)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1,2). Both Stat5a and Stat5b regulate interleukin-7 induced B-cell precursor expansion (3, 4). Stat5b may also act as a transcriptional inhibitor as demonstrated by inhibition of NF-kB mediated signaling (5). This Stat5b-mediated inhibitory effect on NF-kB signaling does not depend on Stat5b-DNA interactions but requires the carboxyl terminus of Stat5b as well as Stat5b nuclear translocation and/or accumulation, suggesting that Stat5b is competing for a nuclear factor(s) necessary for NF-kB-mediated activation of target promoters.
Catalog #S7972-15G
ApplicationsSuitable for the Western blot. Jurkat cell lysate can be used as positive control and protein band of approximate molecular weight of 92kD is detected. Western blot analysis of Stat5b in 10ug of Jurkat cell lysate using 1 ug/m antibody. Immunoreaction was detected by chemiluminescence
Recommended DilutionWestern blot: 1-2ug/ml
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids 771-787 of human Stat5b. This peptide is identical to homologues amino acid sequence in mouse, rat, bovine, monkey and chicken.
SpecificityRecognizes human STAT 5b (Signal Transducers and Activators of Transcription 5b)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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