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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 6, phosphorylated (Tyr641) (STAT6B, STAT6C, D12S1644, IL-4-STAT, Interleukin 4 Induced STAT, Signal Transducer and Activator of Transcription 6)

Anti -STAT 6, phosphorylated (Tyr641) (STAT6B, STAT6C, D12S1644, IL-4-STAT, Interleukin 4
Induced STAT, Signal Transducer and Activator of Transcription 6)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP FC IF
Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFNa in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
Catalog #S7972-25A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (IF-IC): 1:100 requires methanol permeabilization
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues around Tyr641 of human Stat6. Species Sequence Homology: bovine 100%
SpecificityRecognizes endogenous levels of human Stat6 only when phosphorylated at Tyr641 (MW 110kD). Does not crossreact with the corresponding phospho-tyrosine residues of other Stat proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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