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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 6, phosphorylated (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

Anti -STAT 6, phosphorylated (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced
STAT, Signal Transducer and Activator of Transcription 6)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B
Catalog #S7972-46
Applications Suitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.52ug/ml detects phospho-STAT 6 in RIPA lysates from IL4-stimulated HepG2 cells. Unstimulated (lane 1) or IL4 stimulated HepG2 (lane 2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT 6 (Y641), 0.5ug/ml. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i61
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in ~143ul of PBS, 0.09% sodium azide before the addition of glycerol to 30%.
PurityPuri ed by subsequent thiophilic adsoption and size exclusion chromatography.
ImmunogenKLH-conjugated, synthetic phosphopeptide corresponding to amino acids 630650 (KPEQMQKDQRQ[pY]VPATIKMTV) of human STAT 6. The immunizing sequence has 19/21 identical amino acids in mouse.
SpecificityRecognizes phosphorylated STAT6, Mr 100kD. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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