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You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -STAT 6, phosphorylated (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

Anti -STAT 6, phosphorylated (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced
STAT, Signal Transducer and Activator of Transcription 6)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
STAT (signal transducers and activators of transcription) proteins are transcription factors that are selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins have highly conserved amino termini. STAT family members diverge in their carboxy termini. STAT6 is activated by cytokines, IL-4 and IL-3. Evidence shows that STAT6 becomes activated after
PDGF stimulation of NIH 3T3 fibroblasts. STAT6 is distinguishable from the other STAT family members by its ability to bind a GAS-like element found in the Ig germ line e promoter of the IL-4 responsive human Ce gene (Ie).
Catalog #S7972-47
ApplicationsWestern Blotting: 1:1000 dilution detects phospho-STAT6 in lysates of Daudi cells
stimulated with rhIL-4. 100ug of lysate from quiescent (lane 1) or rhIL-4 stimulated Daudi cells (lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT6 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid in PBS with 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids 631-645 of human STAT6 (CPEQMGKDGRGpYVPAT).
SpecificityRecognizes phospho-STAT6, Mr ~100kD; no Crossreactivity with unphosphorylated
STAT6; recognizes a non-specific band at 30kD. Species CrossreactivityHuman and mouse, other species not determined.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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