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You are here:Home » Growth Factors, Cytokines » Growth Factors-Cell Factors » Stem Cell Factor, Recombinant, Human (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor, Recombinant, Human (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast
Cell Growth Factor, MGF)

Pricing

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Specifications

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Human SCF is an 18.4kDa protein containing 164 amino acid residues.
Catalog #S7973
Source E. coli
Form Supplied as a lyophilized powder from 20mM acetic acid. No additives added. Reconstitute with 10mM acetic acid to a concentration of 0.1-1mg/ml
Purity 98% by SDS-PAGE and HPLC analysis.
Endotoxin 0.1ng/ug
Biological Activity Human SCF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of M-07E cells was found to be 20 ng/ml, corresponding to a specific activity of 5x10e4 units/mg.
Responding Cells (partial list) Stem cells, BM precursor cells
Concentration Range for most in vitro applications 0.2-20ng/ml
Sequence MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin US
Molecular Weight18.4kD
SourceE. coli
Purity98% by SDS-PAGE and HPLC analyses.
Concentration~0.1-1mg/ml
FormSupplied as a lyophilized powder from 10mM acetic acid. Reconstitute with ddH2O to a concentration of 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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