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You are here:Home » Molecular Biology » Growth Factors-SCF » SCF, Recombinant, Human (Kit Ligand, Mast Cell Growth Factor, MGF, Stem Cell Factor, c-Kit Ligand, KITLG)

SCF, Recombinant, Human (Kit Ligand, Mast Cell Growth Factor, MGF, Stem Cell Factor, c-Kit
Ligand, KITLG)

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Specifications

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40kD type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189aa extracellular domain (ECD), a 23aa transmembrane segment, and a 36aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25kD soluble molecule which is comparable to the only formproduced by Steel-dickie mutant mice. An alternatively spliced isoform of human SCF exists that lacks 28aa that encompass the primary proteolytic recognition site. Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%-87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
Catalog #S7973-01A
SourceRecombinant corresponding to aa26-189 from human HEK293.
Molecular Weight~18.5kD
Endotoxin ~1EU/1ug (LAL)
Biological ActivityMeasured in a cell proliferation assay using TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 1-5ng/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS and BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.5kD
SourceEmbryonic kidney cell
Purity~95% (SDS-PAGE)
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, BSA. Reconstitute with sterile 100ug/ml PBS, 0.1% BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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