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You are here:Home » Growth Factors, Cytokines » Growth Factors-Cell Factors » Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth
Factor, MGF)

Pricing

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Specifications

Recombinant Human SCF is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
Catalog #S7973-02
SequenceFirst five N-terminal amino acids: Met-Glu-Gly-Ile-Cys.
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 10e5 IU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C..Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.4kD
SourceE. coli
Purity95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Form: Supplied as a lyophilized powder from 0.02% sodium bicarbonate. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration of >0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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