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You are here:Home » Growth Factors, Cytokines » Growth Factors-Cell Factors » Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth
Factor, MGF)

Pricing

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Specifications

Recombinant murine SCF is a single, non-glycosylated polypeptide chain containing 165 amino acids.
Catalog #S7973-12
Amino Acid SequenceMKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
Biological ActivityRecombinant Murine SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cell line is <10ng/ml, corresponding to a Specific Activity of 1x10e5 IU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight1.8309kD
SourceMurine, recombinant protein expressed in E. coli
PurityChromatographically purified ( 98% by SEC-HPLC, SDS-PAGE).
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from 0.02% NaHCO3, pH 6.5.
ReconstitutionReconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration >100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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