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You are here:Home » Growth Factors, Cytokines » Growth Factors-Cell Factors » Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)

Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)

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Specifications

Stem Cell Growth Factor alpha and beta are hematopoietic growth factors that exert their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGFb is a 25kD polypeptide containing 227 amino acid residues.
Catalog #S7973-30
Endotoxin 0.1ng/ug (1EU/ug).
Biological ActivityDetermined by the dose dependant stimulation of the proliferation of human TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed is 7ng/ml.
Amino Acid SequenceMGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Add carrier protein and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Molecular Weight25.0kD
SourceE. coli
Purity98% by SDS-PAGE and HPLC.
FormSupplied as a lyophilized powder, 1mM DTT. Reconstitute with sterile ddH2O to 0.1-1.0mg/ml. DO NOT VORTEX. Add buffer containing carrier protein for long term storage.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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