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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

Anti -Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IC
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2 or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in
S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In
addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2).
Catalog #S7975-11C
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
ConcentrationNot determined
FormAs reported
PurityPurified by immunoaffinity chromatography.
ImmunogenA 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded in part by exon 31 and 32 (LocusLink ID 10735).
SpecificitySpecific for human SA2 protein. Does not react with Xenopus. Other species have not been tested. Reactivity with mouse SA2 is likely owing 32 and 34 identical residues between mouse SA2 and the peptide used for this antibody.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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