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You are here:Home » Growth Factors, Cytokines » Growth Factors-SDF » Stromal Cell Derived Factor 1alpha, Recombinant, Human (SDF1a)

Stromal Cell Derived Factor 1alpha, Recombinant, Human (SDF1a)

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Specifications

Chemokine SDF-1 is highly conserved among species and belongs to a large family of small, structurally related cytokines. It is a chemoattractant for T lymphocytes, monocytes, and hemopoietic stem cells and is constitutively expressed in bone-marrow-derived stromal cells. Recent reports indicate that SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. SDF-1a and SDF-1b, are generated by alternative RNA splicing of transcripts encoded by a single gene. SDF-1a differs from SDF-1b by four C-terminal amino acid residues absent in SDF-1a. As yet, no functional differences have been reported.
Stromal Cell-Derived Factor-1 (SDF1 ) is a recently discovered protein belonging to the -chemokine (C-X-C) family of cytokines. Human SDF1 is an 8kD protein containing 68 amino acid residues.
Recombinant Human SDF-1 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8008 Dalton. The rHuSDF-1 is purified by proprietary chromatographic techniques.
Catalog #S7975-50C
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu
Biological ActivityThe specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80ng/ml.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of SDF-1 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight8.01kD
SourceE. coli
Purity98% by RP-HPLC and SDS-PAGE. Chromatographically purified.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Reconsitute with sterile ddH2O to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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