Stromal Cell-Derived Factor-1 alpha, Recombinant, Human (SDF1A, Stromal Cell-derived
Factor 1, SDF1, SDF-1, hSDF-1, Chemokine (C-X-C motif) Ligand 12, C-X-C Motif Chemokine
12, CXCL12, Intercrine Reduced in Hepatomas, IRH, hIRH, Pre-B Cell Growth-stimulat
Pricing
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| Catalog # | S7975-50E | | Source | Recombinant corresponding to 68aa of human Stromal Cell-Derived Factor-1 alpha expressed in E.coli is a non-glycosylated, polypeptide chain. | | Molecular Weight | ~8kD | | Biological Activity | The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80nug/ml. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 1.06 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SDF-1 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~8kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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