Stromal Cell Derived Factor 1 alpha, Recombinant, Mouse (SDF1 alpha, SDF1a, SDF-1a,
Chemokine (C-X-C Motif) Ligand 12, CXCL12, hIRH, Pre-B cell Growth-stimulating Factor,
PBSF, SCYB12, TLSF-a, TPAR1)
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| Recombinant murine SDF1a is a non-glycosylated, polypeptide chain containing 68 amino acids, Mr 7.9kD. | | | Catalog # | S7975-51A | | Chemokine SDF-1 is highly conserved among species and belongs to a large family of small, structurally related cytokines. It is a chemoattractant for T lymphocytes, monocytes, and hemopoietic stem cells and is constitutively expressed in bone-marrow-derived stromal cells. Recent reports indicate that SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. SDF-1a and SDF-1b, are generated by alternative RNA splicing of transcripts encoded by a single gene. SDF-1a differs from SDF-1b by four C-terminal amino acid residues absent in SDF-1a. As yet, no functional differences have been reported. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu. | | Biological Activity | SDF1a is fully biologically active when compared to standard. The specific activity was determined by its ability to chemoattract human peripheral blood monocytes at 50-100ng/ml. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to | | 100ug/ml by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 7921D | | Source | E. coli | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Endotoxin: 0.1ng/ug (IEU/ug). | | Form | Supplied as a lyophilized powder in 10mM acetic acid (AcOH). Reconstitute in sterile ddH2O to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Stromal Cell Derived Factor 1 alpha, Recombinant, Mouse (SDF1 alpha, SDF1a, SDF-1a, Chemokine (C-X-C Motif) Ligand 12, CXCL12, hIRH, Pre-B cell Growth-stimulating Factor, PBSF, SCYB12, TLSF-a, TPAR1) |
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