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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -Structural Maintenance of Chromosomes 1 Like 1, phosphorylated (Ser957) (SMC1, SMC1L1)

Anti -Structural Maintenance of Chromosomes 1 Like 1, phosphorylated (Ser957) (SMC1,
SMC1L1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B
Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). In response to ionizing radiation (IR), as part of the intra-S-phase DNA damage checkpoint, ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 (3). In cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment, SMC1 phosphorylation is ATM-independent (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra- S-phase checkpoint, the precise mechanismis not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).
Catalog #S7980-01A1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i7
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
PurityAscites
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1.
SpecificityDetects endogenous levels of human SMC1 (145kD) only when phosphorylated at serine 957. May also recognize phosphorylated human DNA-PKcs (450kD). Species Crossreactivity: bovine and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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