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Anti -Structural Maintenance of Chromosomes 1 Like 1, phosphorylated (Ser957) (SMC1, SMC1L1)
Anti -Structural Maintenance of Chromosomes 1 Like 1, phosphorylated (Ser957) (SMC1,
SMC1L1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B |
|
| Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). In response to ionizing radiation (IR), as part of the intra-S-phase DNA damage checkpoint, ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 (3). In cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment, SMC1 phosphorylation is ATM-independent (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra- S-phase checkpoint, the precise mechanismis not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3). | | | Catalog # | S7980-01A1 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 4i7 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. | | Purity | Ascites | | Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1. | | Specificity | Detects endogenous levels of human SMC1 (145kD) only when phosphorylated at serine 957. May also recognize phosphorylated human DNA-PKcs (450kD). Species Crossreactivity: bovine and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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