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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -Structural Maintenance of Chromosomes 1, phosphorylated (Ser957) (SMC1)

Anti -Structural Maintenance of Chromosomes 1, phosphorylated (Ser957) (SMC1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Catalog #S7980-01E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H374
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 951-962 SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.
SpecificityRecognizes phospho-SMC1 (Ser957), Mr 150kD. Species Crossreactivity: Human and mouse. Predicted to crossreact with Xenopus and bovine based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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