Anti -SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3,
Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate
Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IF |
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| The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). | | | Catalog # | S7982-03F | | Applications | Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunofluorescence (IF-IC): 1:200 | | Western Blot: 1:2000 | | Immunoprecipitation: 1:200 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | D47B5 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues surrounding Arg132 of human SMC3. | | Specificity | Recognizes endogenous levels of total human SMC3. Species Crossreactivity: Bovine, chicken, monkey, mouse, rat, xenopus and zebrafish. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1) |
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