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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Substance P (Neurokinin-1), is member of the tachykinin peptide family. Processing of preprotachykinin isoforms generates Substance P and other tachykinins. Active Substance P is an 11 amino acid peptide, RPKPQQFFGLM, that is identical across all mammalian species. Substance P is mainly secreted from C-type fibers of primary sensory afferent neurons after stimulation through protease-activated receptor 2 (PAR2). Substance P binds primarily to the neurokinin 1 receptor on neurons in the dorsal horn, transmitting pain signals through the central nervous system.
Catalog #	S8000-01
Applications	Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 8-25ug/ml. Detects Substance P in paraffin-embedded human spinal cord tissue sections when used with the appropriate secondary reagents.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	7H63
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	KLH-conjugate of Substance P, (RPKPQQFFGLM).
Specificity	Recognizes human Substance P. Species Crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.