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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum IH

Catalog #	S8000-21
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:50-1:100
	Optimal dilutions to be determined by the researcher.
Immunohistochemistry Protocol	Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50ml of Ca2+ free Tyrode’s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1M phosphate buffer, pH 7.4 containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at RT. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours. After rinsing in PBS 3 times sections were incubated for 60 minutes at RT with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid with 0.05% sodium azide.
Purity	Neat serum.
Immunogen	A 12 amino acid peptide corresponding to amino acids 1-11 from rat Substance P.
Specificity	Recognizes rat Substance P. Species crosseactivity: human and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.