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You are here:Home » Antibodies » Abs to Substance P » Anti -Substance P (NK, Neurokinin)

Anti -Substance P (NK, Neurokinin)

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum IH
Catalog #S8000-21
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Immunohistochemistry ProtocolMale Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50ml of Ca2+ free Tyrode’s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1M phosphate buffer, pH 7.4 containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at RT. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours. After rinsing in PBS 3 times sections were incubated for 60 minutes at RT with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceRat
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide.
PurityNeat serum.
ImmunogenA 12 amino acid peptide corresponding to amino acids 1-11 from rat Substance P.
SpecificityRecognizes rat Substance P. Species crosseactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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