SUMF1

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Hu Mo

Accession #

NP_877437.2

Shipping Temp

Blue Ice

Storage Temp

-20°C

SUMF1 activates sulfatases by converting their active site residue cysteine to formylglycine.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:16,000 Western Blot: 0.5-2ug/ml detects ~37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2). Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-ETLNPKGPPSGKDR from the internal region of human SUMF1 (NP_877437.2).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Purity

Purified by affinity chromatography.

Specificity

Recognizes human SUMF1. Species crossreactivity: mouse. Sequence Homology: rat, bovine, canine.

References

1. Oshikawa M, Usami R, Kato S. Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19. Molecular vision 2009 15 : 482-94. PMID: 19262745